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hind iii  (New England Biolabs)


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    New England Biolabs hind iii
    Hind Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3554 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hind iii/product/New England Biolabs
    Average 96 stars, based on 3554 article reviews
    hind iii - by Bioz Stars, 2026-03
    96/100 stars

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    (A) (i) The PbHiT vector is linearised using BsmBI and <t>PstI</t> <t>restriction</t> enzymes and then (ii) the gRNA-HDR fragment is ligated into the PbHiT vector. (B) Successful ligation is confirmed using colony PCR, where the vector only produces a band around 300 bp, and successful ligation shows a band around 600 bp.
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    (A) (i) The PbHiT vector is linearised using BsmBI and PstI restriction enzymes and then (ii) the gRNA-HDR fragment is ligated into the PbHiT vector. (B) Successful ligation is confirmed using colony PCR, where the vector only produces a band around 300 bp, and successful ligation shows a band around 600 bp.

    Journal: Bio-protocol

    Article Title: Plasmodium berghei High-Throughput (PbHiT): a CRISPR-Cas9 System to Study Genes at Scale

    doi: 10.21769/BioProtoc.5572

    Figure Lengend Snippet: (A) (i) The PbHiT vector is linearised using BsmBI and PstI restriction enzymes and then (ii) the gRNA-HDR fragment is ligated into the PbHiT vector. (B) Successful ligation is confirmed using colony PCR, where the vector only produces a band around 300 bp, and successful ligation shows a band around 600 bp.

    Article Snippet: PstI restriction enzyme (New England Biolabs, catalog number: R0140L) 5.

    Techniques: Plasmid Preparation, Ligation